A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes

Julien Dumont; Sebastian Petri; Franz Pellegrin; Marie-Emilie Terret; Markus T. Bohnsack; Pascale Rassinier; Virginie Georget; Petr Kalab; Oliver J. Gruss; Marie-Hélène Verlhac

doi:10.1083/jcb.200605199

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A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes

Article de revue scientifique

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A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes

Julien Dumont, Sebastian Petri, Franz Pellegrin, Marie-Emilie Terret, Markus T. Bohnsack, Pascale Rassinier, Virginie Georget, Petr Kalab, Oliver J. Gruss et Marie-Hélène Verlhac

The Journal of cell biology, Vol.176(3), p.295-305

29/01/2007

DOI: https://doi.org/10.1083/jcb.200605199

PMID: 17261848

Résumé

Spindle formation is essential for stable inheritance of genetic material. Experiments in various systems indicate that Ran GTPase is crucial for meiotic and mitotic spindle assembly. Such an important role for Ran in chromatin-induced spindle assembly was initially demonstrated in Xenopus laevis egg extracts. However, the requirement of RanGTP in living meiotic cells has not been shown. In this study, we used a fluorescence resonance energy transfer probe to measure RanGTP-regulated release of importin β. A RanGTP-regulated gradient was established during meiosis I and was centered on chromosomes throughout mouse meiotic maturation. Manipulating levels of RanGTP in mice and X. laevis oocytes did not inhibit assembly of functional meiosis I spindles. However, meiosis II spindle assembly did not tolerate changes in the level of RanGTP in both species. These findings suggest that a mechanism common to vertebrates promotes meiosis I spindle formation in the absence of chromatin-induced microtubule production and centriole-based microtubule organizing centers.

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Titre: A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes
Créateurs - sans rôle: Julien Dumont - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Sebastian Petri - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Franz Pellegrin - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Marie-Emilie Terret - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Markus T. Bohnsack - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Pascale Rassinier - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Virginie Georget - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Petr Kalab - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Oliver J. Gruss - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Marie-Hélène Verlhac - UMR7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany Institut de Biologie Intégrative, IFR83, 75005 Paris, France Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
Détails de publication: The Journal of cell biology, Vol.176(3), p.295-305
Éditeur: The Rockefeller University Press
Nombre de pages: 11
Identifiants: 99234050309311
Unité académique: CRBM - Centre de Recherche en Biologie Cellulaire de Montpellier
Langue: Anglais
Type de ressource: Article de revue scientifique