Résumé
Mass spectrometry has become indispensable in studying post-transcriptional modifications of RNA, a.k.a. epitranscriptomics, which are crucial for regulating RNA metabolism, gene expression, and major biological processes. Over 150 chemical modifications have been identified across all RNA subtypes. Dysregulation of RNA mark deposition can lead to disease development and progression. Therefore, accurately detecting and characterizing these modifications are vital for understanding their functional impact and uncovering potential therapeutic and diagnostic biomarkers. In this chapter, we describe a mass spectrometry-based method for detecting and quantifying nearly 40 different RNA modifications.