A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline

Vasily V. Vagin; Alla Sigova; Chengjian Li; Hervé Seitz; Vladimir Gvozdev; Phillip D. Zamore

doi:10.1126/science.1129333

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A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline

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A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline

Vasily V. Vagin, Alla Sigova, Chengjian Li, Hervé Seitz, Vladimir Gvozdev et Phillip D. Zamore

Science (American Association for the Advancement of Science), Vol.313(5785), pp.320-324

21/07/2006

DOI: https://doi.org/10.1126/science.1129333

PMID: 16809489

Résumé

In the Drosophila germline, repeat-associated small interfering RNAs (rasiRNAs) ensure genomic stability by silencing endogenous selfish genetic elements such as retrotransposons and repetitive sequences. Whereas small interfering RNAs (siRNAs) derive from both the sense and antisense strands of their double-stranded RNA precursors, rasiRNAs arise mainly from the antisense strand. rasiRNA production appears not to require Dicer-1, which makes microRNAs (miRNAs), or Dicer-2, which makes siRNAs, and rasiRNAs lack the 2′,3′ hydroxy termini characteristic of animal siRNA and miRNA. Unlike siRNAs and miRNAs, rasiRNAs function through the Piwi, rather than the Ago, Argonaute protein subfamily. Our data suggest that rasiRNAs protect the fly germline through a silencing mechanism distinct from both the miRNA and RNA interference pathways.

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Titre: A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline
Créateurs - sans rôle: Vasily V. Vagin - Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Department of Animal Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia
Alla Sigova - Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Department of Animal Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia
Chengjian Li - Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Department of Animal Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia
Hervé Seitz - Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Department of Animal Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia
Vladimir Gvozdev - Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Department of Animal Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia
Phillip D. Zamore - Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Department of Animal Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia
Détails de publication: Science (American Association for the Advancement of Science), Vol.313(5785), pp.320-324
Nombre de pages: 5
Identifiants: 99159294709311
Unité académique: Institut de Génétique Humaine - IGH
Langue: English
Type de ressource: Journal article