Abstract
While nucleic acids are known to be versatile biomolecules that play a crucial role in regulating gene expression, the predictable nature of nucleic acid hybridization offers a simple and cutting-edge platform to program assemblies with emerging function. The burgeoning use of nucleic acids as a material to organize the precise arrangements of specific molecules marked an important milestone in the relatively recent history of nanotechnologies and bioanalysis.In the first part of this work, the 10-23 DNAzyme has been choosed as a model to develop and study new boronic acids-containing nucleic acid-based functional systems able to reversibly self-assemble responding to an external stimuli. Through this study we have developed new supported conjugation methods allowing the incorporation of boronic acids or aldehydes functions into nucleic acids sequences. Cyclic oligonucleotides bound by boronate esters junctions and formed by self-assembly were also described in a third part. Finally, in order to diversify internucleosidic bounds available, the fourth part of this work allowed the elaboration of new internucleosidic junctions based on oxazaborolidines.