Abstract
Transcription in eukaryotes is highly complex process that requires precise coordination in the assembly of trans-regulating factors through the recognition of cis-regulatory DNA sequences. By employing quantitative live imaging methods combined with a mathematical deconvolution model, we assess the influence of the core promoter DNA sequences on transcription dynamics in vivo. Furthermore, manipulation of TATA box from a robust developmental core promoter: snail, in a transgenic context showed its influence to foster nuclei activation within the pattern. Moreover, strong mutation of the TATA does not lead to promoter death, suggesting an inherent potential of this promoter to allow permissive transcription. In contrast, mutations of the INR motif in the kruppel transgenic core promoter does not change the timing of nuclei activation; neither the probability of nuclei activation, but rather the INR mutations seems to affect the promoter strength. We show in this study that core promoter sequence might act as a key step to regulate gene expression, and its element composition might be adapted to particular gene context.