Abstract
Iron is a microelement crucial for the function of a large number of biological way. One of the iron assimilation products is the iron sulphur (Fe-S) cluster. These cofactors are implicated in various essential reaction like photosynthesis or mitochondrial respiration. A large amount of protein’s families is involved in the Fe-S proteins maturation, among them, there is the BOLA family. My research focus on the function of two member of the BOLA family in plants called BOLA1 and BOLA4. By transcriptomic approach and transgenic lines study with rapporteur genes, I could show that BOLA1 and BOLA4 genes have a constitutive expression profile and there are presents in both plastids and mitochondria. After the creation and characterization of the bola1 bola4 double-mutant, I demonstrated that BOLA1 and BOLA4 proteins have a functional redundancy and are very important in plant growth. The phenotypic characterization, coupled with proteomic approach, showed that BOLA1 and BOLA4 are involved in the root architecture maintenance, long-distance signalling, carbon assimilation and iron homeostasis regulation. It was also observed that BOLA1 and BOLA4 are implicated in the maintenance of many biological ways involving Fe-S cluster proteins. These results bring a better comprehension of the functions of BOLA family and describe for the first time a role of BOLA1 and BOLA4 in plants.