Abstract
Chronic wounds are a severe public health challenge affecting 2% of the global population. Their prevalence is constantly increasing, along with population aging and obesity. Diabetic foot ulcer (DFU) particularly is constantly rising as the number of people with diabetes mellitus is also rising every year. Wound impairment in DFU is associated with biofilm. Biofilm induces wound infection and promotes antibiotic resistance. Therefore, instead of antibiotics, alternative therapeutic options are crucially needed in DFU treatment.Antiseptic agents are one of the alternative options in DFU care. However, there are few studies documenting antiseptic efficiency in chroming wound healing and a lack of evidence on recommending one antiseptic over another.This thesis work focused on biofilm models adaptation for evaluating antiseptic agents on monospecies and bispecies biofilms using clinical isolates of the most prevalent strains isolated from DFU: S.aureus and P. aeruginosa. Evaluation was proceeded at 3 levels. First on free planktonic bacteria, next on biofilm formation inhibition, then on living bacterial load reduction and finally on a biofilm post debridement in a dynamic flow model; like clinical practice.These works allowed the identification of Sodium hypochlorite as a relevant antibiofilm agents In DFU treatment compared to PolyHexaMethyleneBiguanide and Octenidine.