Résumé
This deposit contains all of the processed data and analysis scripts supporting the findings in “Tunable simultaneous propulsion by two distinct motility motors drives surface motility in bacteria.”
/Data.zip
Contains all processed cells trajectories and focal-adhesion datasets used to generate figures and quantitative analyses in the manuscript.
/Cells_data
Agar_0p75: pickle files with per‐cell, per‐frame (x,y) trajectories for leading pole, lagging pole, and center on 0.75 % agar.
Ibidi_chitosan: same format for trajectories recorded on chitosan-coated coverslips.
Umap: two-dimensional embeddings of the 48 motility features for all strains.
/FACS_data
Agar_predation: detected focal-adhesion complexes (FACS) from AglZ-YFP in predation assays; one .pkl per time‐lapse.
Ibidi_chitosan: FACS spot coordinates for both A⁺S⁻ and WT strains on chitosan substrates.
/Scripts.zip
Includes all code and configuration files required to reproduce data processing, analysis, and figure generation:
config/parameters.jsonDefines data paths and processing and analysis settings.
modules/Python modules implementing image preprocessing, segmentation, tracking, feature extraction (kymographs, pole detection), FACS detection, and trajectory feature computation.
run_pipeline.pyOrchestrates raw data ingestion, processing through most modules for cell tracking, and exports the final .pkl datasets.