Résumé
The alpine plant Arabis alpina is an emerging model in the ecological
genomic field which is well-suited to identifying the genes involved in
local adaptation in contrasted environmental conditions, a subject which
remains poorly understood at molecular level. This paper presents the
assembly of a pool of A. alpina genomic fragments using Next Generation
Sequencing technologies. These contigs cover 172 Mb of the A. alpina
genome (i.e. 50% of the genome) and were shown to contain sequences giving
positive hits against 96% of the 458 CEGMA core genes (Core Eukaryotic
Genes Mapping Approach), a set of highly conserved eukaryotic genes.
Regions presenting high nucleic sequence identity with 77% of the close
relative Arabidopsis thaliana's genes were found, with an unbiased
distribution across the different functional categories of A. thaliana
genes. This new resource was tested using a resequencing assay to identify
polymorphic sites. Sixteen samples were successfully analyzed and 127,041
Single Nucleotide Polymorphisms identified. This contig dataset will
contribute to improving understanding of the ecology of Arabis alpina,
thus constituting an important resource for future ecological genomic
studies.