Résumé
The emergence of pathogens represents substantial threats to public
health, livestock, domesticated animals, and biodiversity. How wild
populations respond to emerging pathogens has generated a lot of interest
in the last two decades. With the recent advent of high-throughput
sequencing technologies it is now possible to develop large transcriptomic
resources for non-model organisms, hence allowing new research avenues on
the immune responses of hosts from a large taxonomic spectra. We here
focused on a wild population of the rostrum dace (Leuciscus burgiladensis)
that is infected by Tracheliastes polycolpus, an emerging freshwater
ectoparasite copepod. We used next generation Illumina sequencing
technology to sequence the transcriptome of eight L. burdigalensis adult
individuals collected in natura from the same sampling site. Four
individuals were non-infected and four individuals were infected by T.
polycolpus. We specifically focused on the spleen, the head kidney and
epithelial cells and mucus from the fins, three tissues known to be
involved in the immune response of fish. We used the Trinity methodology
to reconstruct a de novo full-length transcriptome for L. burdigalensis.
The resulting transcriptome will serve as an important broad-scale genomic
resource for further studying the response of local population of L.
burdigalensis to T. polycolpus pressures.