Résumé
Astec-Pm13 Live embryonic development of Phallusia mammillata (Phlebobranch ascidian).
Cell membranes were fluorescently labeled by microinjection of mRNA encoding PH-tdtomato and KTR-Clover into unfertilized egg. The embryo has been live imaged with a MuVi-SPIM for three hours, from the 25-cell stage (Tp 0, ~St. 6) up to the 231-cell mid Gastrula stage (Tp 89, St. 12), at 18°C.
Whole cells were segmented using the ASTEC pipeline.
* The four angle fusion images obtained using the ASTEC pipeline are shared in .inr format (fuse.tar.gz archive file).
* Whole cells were segmented using the ASTEC pipeline and shared in .inr format (post.tar.gz archive file).
* Individual cell geometric properties are shared in pkl formats (properties.tar.gz archive file).