Résumé
Asaspe-1Organism: Ascidiella aspersa embryo (Phlebobranch ascidian)Description:This dataset contains live imaging data of embryonic development in Ascidiella aspersa. The cell membranes were fluorescently labeled by microinjection of mRNA encoding PH-tdTomato into an unfertilized egg.Imaging conditions:The embryo was imaged live with a Luxendo MuVi-SPIM light-sheet microscope from the 32-cell stage (Tp0, St. 6) up to the mid neurula stage (Tp124, St. 15). Acquisitions were performed every 2 minutes at 18 °C in artificial seawater (ASW, salinity: 38 ppt).Temporal alignment:Embryonic development in ascidians shows natural variability in timing between individuals. To enable direct comparison across datasets, developmental time was normalized based on cell number. Phmamm-8 (Phallusia mammillata) was chosen as the reference embryo, and Asaspe-1 was temporally aligned to it. In the normalized timeline, the acquisition of Asaspe-1 corresponds to a starting point (t0) of approximately 200 minutes post fertilization (tpf), with an effective acquisition interval of 1.72 minutes. This temporal alignment was performed using the ASCIDIAN package (see documentation for details). This allows stage-by-stage comparison of Asaspe-1 with Phmamm-8 and the other ascidian embryo datasets.Data processing:Fusion of images from four angles, whole-cell segmentation and temporal registration to correct for embryo movement were performed using the ASTEC pipeline (detailed documentation available here).Cell identification and fate assignments were executed using the ASCIDIAN package (documentation available here).Dataset versions:Biasuz et al., 2025 (v3)Fused and temporally registered images (.nii format): Available as "Asaspe-1-v3_intrareg_fuse.tar.gz".Intraregistered segmented images compensating embryo movements (.nii format): Available as "Asaspe-1-v3_intrareg_post.tar.gz".Individual cell geometric and naming properties (.xml format): Available as "Asaspe-1-v3-properties.tar.gz".For additional details about the .nii format, please refer to this resource.