Résumé
The plant-parasitic nematode Meloidogyne graminicola causes considerable damages to rice (Oryza sativa) culture. Resistance to M. graminicola in the related species Oryza glaberrima reduces root penetration by juveniles and stops further nematode development. M. graminicola genes expressed during O. sativa (Nipponbare) and O. glaberrima (TOG5681) infection were characterized at different time after infection. M. graminicola RNA-Seq data were retrieved from 14 (7 × 2 replicates) cDNA libraries obtained from pre-parasitic M. graminicola stage-two juveniles (J2s), and from O. sativa cv Nipponbare (susceptible) or O. glaberrima TOG5681 (resistant) plants challenged with M. graminicola. Root tips and galls, when formed, were collected at 2, 4, or 8 dpi. Time points 2, 4, and 8 dpi were chosen to focus on the early stages of the interaction, when gene expression differences may determine the nature of interaction between both partners. We generated a de novo M. graminicola transcriptome composed of 44,137 sequences. We compared the M. graminicola transcriptomes before and during infection to identify M. graminicola genes differentially expressed at 2, 4 and 8 days after infection of susceptible (Nipponbare) or resistant (TOG5681) rice.