Résumé
Disease symptoms in T0 transgenic plants over-expressing OsWAK91 or OsWAK112d. Unique rice T0 plants over-expressing OsWAK91 (A, B, C; 5 lines starting with “OE”) or OsWAK112d (D, E, F; 6 lines starting with “OX”) were produced. Plants transformed with the empty vector are also shown (A; 4 lines starting with “EV”). The transgene expression level was normalized using Actin and is expressed as Arbitrary Unit (A, C). The values presented are unique values since each represents a unique T0 plant. Given the extremely low expression levels and thus variability in empty vectors, the very high values in over-expressor lines was considered as significant. Plants were inoculated with the virulent isolate FR13 of Magnaporthe oryzae and lesion number was quantified 7 dpi (B, E). Examples of symptoms are also shown (C, F). The experiments in panels A/B and C/D have not been conducted at the same time but in two separate experiments. The difference between empty vectors can thus be attributed to differences in the inoculum pressure (higher in the case of A/B). (PDF 2113 kb)