Résumé
Wak insertion mutants. A. For each OsWAK gene, the different splicing forms are shown as well as the position of the T-DNA insertion site. The small arrows designate the primers (Additional file 6) that were used to genotype the plants and the primers used for measuring gene expression by quantitative RT-PCR. B. Transcript levels for the corresponding gene as measured by quantitative RT-PCR in mutant plants (MUT) compared to the corresponding null-segregant (WT) plants. Gene expression was normalized using actin. The values represent the percent of gene expression as compared to WT (100Â %); the values are the mean and standard deviation calculated from three biological replicates. (PDF 97 kb)