Résumé
In vitro treatment of CD4+ T cells with DMSCs interferes with Th17 phenotype definition. CD4+ cells purified from C57BL/6 mice spleens were stimulated in vitro by anti-CD3/anti-CD28 antibodies under nonpolarizing condition (A) or under pro-Th17 pressure in the presence of IL-6 and TGFβ (B). Three-day cultures were analyzed for IL-17 expression. Soluble IL-17 measurements were quantified by ELISA. Each sample was assayed in quintuplicate and significance was analyzed by t-test; standard error of the means are shown. Results are shown from one representative out of three independent experiments. (PDF 17 kb)