Résumé
Foxp3 expression by T cells from untreated or DMSC-treated EAE mice (n = 9/group). Spleen cells from EAE animals were obtained at day 10 p.i. Total RNA samples were obtained from purified CD4+ cells and used to quantify Foxp3 mRNA by RT-qPCR (A). Total spleen populations from individual mice were analyzed by cytometry analysis of surface and intracellular staining with anti-CD4-APC and anti-Foxp3-PE, respectively (B). Percentages of CD4+Foxp3+ cells are shown by representative flow dot plots and by the average of the values obtained for each individual mouse from each group. Standard error of the means are shown. (PDF 150 kb)