Résumé
Proton homeostasis is known for a long time as essential for plant cell functions especially in the acidic growth theory that was first proposed almost 50 years ago. More recently, the development of powerful fluorescent microscopes together with an exponentially growing set of pH sensors has opened a new era of real time imaging to study proton distribution at high time and scale resolution. In my talk, I will focus on ratiometric fluorescent sensors for pH that are genetically encoded. I will describe the potential and limitation of such sensors through the examples of protein trafficking in plant cells, ionic exchange between plant root and its environments. Finally I will detail our current and further research project where pH sensors are used to study cell elongation processes.