Résumé
We have designed a new solution-phase approach for oligonucleotide synthesis employing solid-supported reagents which may be recycled. A PolyVinylPyridinium resin (tosylate salt) replaces the classical tetrazole during the coupling step. The sulfurization is performed with a proprietary polystyrene-bound trimethylammonium tetrathionate replacing the Beaucage's reagent. Similarly, oxidation to phosphotriesters was performed with resin as a periodate salt. The use of such supported reagents avoids tedious and expensive purification steps as the excess of reagents are easily removed by filtration.