Reduced photobleaching of BODIPY-FL: SCIENCE AND APPLICATIONS-HBSM 2009

David Dulin; Antoine Le Gall; Karen Perronet; Nicolas Soler; Dominique Fourmy; Satoko Yoshizawa; Philippe Bouyer; Nathalie Westbrook

doi:10.1016/j.phpro.2010.01.222

Acte de colloque

Reduced photobleaching of BODIPY-FL: SCIENCE AND APPLICATIONS-HBSM 2009

David Dulin, Antoine Le Gall, Karen Perronet, Nicolas Soler, Dominique Fourmy, Satoko Yoshizawa, Philippe Bouyer et Nathalie Westbrook

Physics procedia, Vol.3(4), pp.1563-1567

Physics Procedia

01/01/2010

DOI: https://doi.org/10.1016/j.phpro.2010.01.222

Résumé

Instruments & Instrumentation

Physical Sciences

Physics

Physics, Applied

Science & Technology

Spectroscopy

Technology

We present a study of the photophysical properties of the BODIPY-FL fluorescent dye at the single molecule scale using total internal reflexion fluorescence microscopy. We characterized the saturation intensity and the maximum number of fluorescence photons for single BODIPY-FL in a usual buffer. We then studied the influence of the buffer depletion in oxygen and the addition of redox agents on the brightness and the lifetime before photobleaching of the BODIPY-FL. The great improvement of these two factors in such a medium allows the use of BODIPY-FL in single-molecule biophysics experiments. (C) 2010 Elsevier B. V. All rights reserved

Indicateurs

1 Consultations de la notice

Détails

Titre: Reduced photobleaching of BODIPY-FL
Créateurs - sans rôle: David Dulin - CNRS, Inst Opt, Lab Charles Fabry, Campus Polytech,2 Ave Fresnel, F-91127 Palaiseau, France
Antoine Le Gall - CNRS, Inst Opt, Lab Charles Fabry, Campus Polytech,2 Ave Fresnel, F-91127 Palaiseau, France
Karen Perronet - CNRS, Inst Opt, Lab Charles Fabry, Campus Polytech,2 Ave Fresnel, F-91127 Palaiseau, France
Nicolas Soler - CNRS, ICSN, Lab Chim Biol Struct, F-91190 Gif Sur Yvette, France
Dominique Fourmy - CNRS, ICSN, Lab Chim Biol Struct, F-91190 Gif Sur Yvette, France
Satoko Yoshizawa - CNRS, ICSN, Lab Chim Biol Struct, F-91190 Gif Sur Yvette, France
Philippe Bouyer - CNRS, Inst Opt, Lab Charles Fabry, Campus Polytech,2 Ave Fresnel, F-91127 Palaiseau, France
Nathalie Westbrook - CNRS, Inst Opt, Lab Charles Fabry, Campus Polytech,2 Ave Fresnel, F-91127 Palaiseau, France
Contributeurs - sans rôle: H Riesen
M Sellars
N Manson
Détails de publication: Physics procedia, Vol.3(4), pp.1563-1567
Publications en série: Physics Procedia
Éditeur: Elsevier
Nombre de pages: 5
Note de subvention: Centre de Competence Nanosciences de l'Ile de France (C'Nano IdF)
Identifiants: 99136171109311
Unité académique: Centre de Biologie Structurale - CBS
Langue: English
Type de ressource: Conference proceeding