Résumé
Phenolic compounds represent a large family of grape secondary metabolites, essential for thequality of grape and wine and playing a major role in plant defense against biotic and abioticstress. Phenolic composition is genetically driven but also greatly affected by environmentalfactors and in particular by drought. A major challenge for selection of grapevine cultivarsadapted to climate change and with high potential for winemaking is to dissect the complex plantmetabolic response involved in adaptation mechanisms.A targeted metabolomics approach based on UPLC-QqQ-MS analysis in the MRM mode(Lambert et al., 2015) has been developed for high throughput profiling of the phenoliccomposition of grape skins. This method enables rapid, selective, and sensitive quantification of96 phenolic compounds (anthocyanins, phenolic acids, stilbenoids, flavones, flavanones, flavan-3-ol monomers and oligomers…), and of the constitutive units of proanthocyanidins (i.e. flavan-3-ololigomers and polymers, also called condensed tannin), giving access to detailed polyphenolcomposition.It has been applied on the skins of mature berries from a core-collection of 279 V. viniferacultivars grown with or without watering to assess the genetic variation for polyphenolcomposition as a response to differential water availability, in the frame of the EU projectInnovine. Chemometrics analysis of the phenolic composition data has shed light on the geneticdiversity of vine metabolic response to drought.