Résumé
The genome of banana (Musa sp.) harbours multiple integrations of Banana streak virus (BSV), whereas this badnavirus does not require integration for the replication of its ds DNA genome. Some endogenous BSV sequences (eBSV), only existing in the Musa balbisiana genome, are infectious by releasing a functional viral genome following stresses such as those existing in in vitro culture and interspecific crosses context. The structure of these eBSV is much longer than a single BSV genome, composed of viral fragments duplicated and more or less extensively rearranged. Wild M. balbisiana diploid genotypes (BB) such as Pisang Klutuk Wulung (PKW) harbour such infectious eBSV belonging to three widespread species of BSV (Goldfinger -BSGFV, Imové - BSImV and Obino l'Ewai - BSOLV) but are nevertheless resistant to any multiplication of BSV without any visible virus particles. We postulated these eBSV induced a natural resistance driven by gene silencing mechanisms based on their complex molecular re-arranged structure which could lead to dsRNA hairpins formation. In collaboration with the group headed by M. Pooggin (Basel, Switzerland), a deep sequencing of total siRNAs of PKW was performed using the Illumina ultra-high-throughput technology. We obtained for the first time, experimental evidence of virus-derived small RNA (vsRNA) from BSOLV, BSGFV and BSImV by blasting sequences against the 3 BSV species genomes. vsRNA are enriched in 24-nt class thus eBSV in PKW genome are likely silenced at the transcriptional level. A repartition of the vsRNA population matching eBSV will be also presented in order to determine hot and cold spots of vsRNA generation. (Texte intégral)