Résumé
Temporal series of 3D images is nowadays a standard for the study of evolving phenomena (eg developmental biology). Multiple microscopy modalities can be used for this purpose: two-photon excitation microscopy, lightsheet microscopy, spinning disk confocal microscopy. Among these, the multiview lighsheet imaging allows to reconstruct a better resolved image by combination of several acquisitions. However, in this particular modality, the stage rotation may cause an extreme motion of the imaged sample, which, in turn, may impair either the fusion of the acquisition or the tracking of individual cells in the temporal series. We present here a methodology to retrieve these extreme motions, which enabled to reconstruct a stabilized temporal series of the fused images.