Résumé
s a key component of the plant’s life cycle since it carries the male gametes forfecundation. Ensuring an optimal pollen production is crucial for both the fitness of the plant(i.e. the transmission of its gene pool to the next generation) and the agronomical yield (seedand/or fruit production). The acquisition and storage of essential metals (Fe, Cu, Zn, Mn…) isconsidered to be crucial for the quality of pollen grains. Therefore, it is strategic to developanalytical approaches to analyze metal homeostasis in pollen grains that are constituted of asingle cell. The quantification of metals in pollen is extremely challenging since the amount ofpollen produced by plants is generally very small. Nevertheless, pollen grains can be easilycollected from flowers, with a high degree of purity. Therefore, this biological material isparticularly well suited to implement single-cell approaches to study metal homeostasis.