Résumé
Casein micelles (CMs) are colloidal protein-mineral complexes in equilibrium in the milk serum. Their enzymatic cross-linking by transglutaminase (TGase) allow a modification of their characteristics. The aim of this work is to determine the impact of this modification on their characteristics (size and elastic properties) in a native liquid environment by atomic force microscopy (AFM). The challenge is to immobilize the particles on a surface in their natural state in order to investigate the effect of covalent cross-linking in a simulated milk ultrafiltrate (SMUF).