Résumé
Pathogenesis related-proteins 10 (PR-10) have ribonuclease and antifungal activity. A PR-10 from Theobroma cacao ( TcPR-10 ) identified in a cDNA library from the cacao- Moniliophthora perniciosa interaction showed antifungal activity against M. perniciosa and Saccharomyces cerevisiae , and the TcPR-10 was internalized by both organisms. Previous studies indicated that the antifungal activity and internalization of TcPR-10 appears to be related to Snq2 membrane permeates and transporters. In order to investigate the antifungal action of TcPR-10 and its internalization in fungal cells, molecular docking was performed to check the interaction between the ABC (Snq2) transporter and TcPR-10 mut, recombinant mutant protein with reduced allergenicity potential. The three-dimensional structure of TcPR-10 mut and Snq2 were modelled and used in molecular docking analysis using the ClusPro online toolkit. Snq2 interacted with TcPR-10 mut in its transmembrane domain, suggesting the internalization of this protein by means of this transporter, similarly to the transport of alkaloids.