Résumé
Ganoderma boninense is a telluric lignicolous basidiomycete and the main causal agent of the basal stem rot, one of the most devastating diseases of oil palm (Elaeis guinensis). While the fight against G. boninense should be a priority in South-East Asia, only scarce information is available about the diversity level of this fungus, and almost nothing is known about its genetic structure and history. In this context, the development of an informative molecular marker set for characterizing G. boninense diversity is a key step to understand the biology of this pathogen. A G. boninense draft genome sequence assembly of 61.5 Mb (from 454 and Illumina sequencing) has been used to identify and develop a set of microsatellite markers (SSR). A total of 652 SSR were identified of which 145 SSR primer sets were developed. These SSR are characterized by motif from 2 to 6 bases long and 5 to 34 repetitions. A total of 97 SSR were successfully amplified on a first small set of G .boninense isolates from Indonesia. Then a population of 48 isolates from several locations in South-East Asia was screened to characterize each locus for allele number, heterozygoty and null allele absence. These results allow us to propose an efficient SSR set to study G boninense in infected oil palm plantations in order to better understand the history of this pathogen. (Résumé d'auteur)