Résumé
Calcium phosphate apatites are naturally present in many biomineralizations of living organisms [1]. They mainly compose the mineral part of bones and teeth. Thanks to their biocompatibility and submicron to nanoscale dimensions, bio-inspired apatites are widely developed for bone engineering and studied more recently for nanomedicine applications (hematology, oncology, dermatology …)[2], often in association with therapeutic agents, to potentially reduce drug toxicity, to avoid the metabolization of some active agents or to improve biodistribution to a specific target.In this presentation, we report how we have combined for the first time peptides and apatites, to develop bioactive colloidal hybrid NPs. Previous studies have shown that added stabilizing agents such as phosphonated PEGs in the synthesis, allowed obtaining colloidal NPs. Based on this principal, we prepared by one-pot synthesis several pegylated peptide-decorated colloidal hybrid NPs. The phosphonate present as oxoanion adsorbs on the apatitic surface, presumably via direct interaction with Ca2+ surface ions. We succeeded to obtain stable and monodisperse colloidal suspensions of rod-shaped apatite crystals with nanoscale dimensions around 30 nm in width and 90 nm in length, and exposing biologically active peptides. The grafting rate obtained by 31P eretic NMR method was estimated at 0.5 molecule/nm2. These data are essential for the biological evaluation of our NPs, especially in the field of dermatology for the treatment of complex wounds.