Résumé
The eriophyid mite Colomerus vitis is the vector of the Grapevine Pinot Gris virus (GPGV), a phytovirus emergent in France and in Europeassociated with Grapevine Leaf Mottling and Deformation disease. Previous studies considering populations from South Africa, Iran, Egypt, Israel, Spain and USA showed that this mite constitutes a complex of cryptic species, including five genetic lineages. Frenchpopulations have not been characterized to date. In France, the status of GPGV is contrasting among regions, possibly due to differences in virus transmission efficiency among eriophyid mite genetic lineages. The aims of this study were to i) characterize the genetic diversity of Col. vitis from France; and ii) check for a relation between the genetic pool of Col. vitis and the incidence of GPGV. For this, populations from 44 vineyards on 17 departments were sampled. For each plot, around 10 specimens from 5 vines were isolated for DNA extraction and COI sequencing. GPGV rt-PCR tests were conducted to detect the virus in sampled vines. A total of 574 sequences were obtained. Phylogenetic analysis, which datasets included all public sequences available in GenBank and previously obtained by the team, revealed the co-occurrence of the five divergent COI genetic lineages in France, with two dominant lineages - G1 and G5. Statistical analysis suggests a significant association between the presence of GPGV and group G1 within a vine (p=0.02), indicating that G1 is most strongly associated with virus transmission. Further studies should investigate the efficiency of virus transmission by the two dominant lineages.