Résumé
A wide range of proteins viewed using xFP probes in fluores- cence video-microscopy shows a cytosolic state diffusing slowly and a membrane state eventually moving as vesicles or small tubules along the cell cytoskeleton network. Due to the property of fluores- cence, the measured intensity is the sum of the contributions of these two components. In this study we perform an evaluation of different methods for the automatic decomposition of the two additive com- ponents/states. Four algorithms are studied: i) gray-scale opening (a.k.a. "rolling ball"); ii) wavelet-based detection combined with image interpolation; iii) Conditional Random Field-based detection; iv) computational geometry for temporal signal analysis. The eval- uation protocol, related to the actual use of image sequence decom- position, includes a qualitative evaluation on real image sequences by experts and a quantitative evaluation on simulated sequences that mimic real images.