Résumé
Regenerative medicine brings a huge application for Mesenchymal stem cells such as Dental Pulp Stem Cells (DPSCs). Confocal Raman microscopy, a non-invasive, label free, real time and high spatial resolution imaging technique is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800-3000 cm(-1) region (C-H stretching) and 960 cm(-1) peak (phosphate PO43-) were collected. In Dental Pulp Stem Cells 21st day differentiated in buffer solution, phosphate peaks nu(1) PO43- (first vibrational mode) at 960cm(-1) and nu(2) PO43- at 430cm(-1) and nu(4) PO43- at 585cm(-1) are obviously present. Confocal Raman microscopy enables the detection of cell differentiation and it can be used to investigate clinical stem cell research.