Résumé
This paper presents the metabolic-fluxes calculation of a metabolic network representing thecentral metabolism of the yeast Saccharomyces cerevisiae in the wine-making context. Two solutionmethods are compared: a metabolic flux analysis (MFA) using convex analysis and providing narrowintervals of variation for the fluxes, and a flux balance analysis (FBA) based on an objective function.The constraints allowing the solution of the underdetermined set of algebraic equations are typicallyoriginating from measurements of uptake and secretion rates of external metabolites, and/or the use of anobjective function, and/or metabolic constraints. It is shown here that converting reactions schemes inCmol unit combined with data reconciliation provides a convenient formulation of closed carbon balance.In this form, the constraint formulation is natural and facilitates the understanding of the carbondistribution within the yeast metabolism.