Résumé
WARDA initiated research on rice anther culture in 1993 when it produced its first doubled haploid lines from F1, F2 and F3 progenies. Objectives include production of doubled haploid lines from japonica X japonica, indica X indica and indica X japonica crosses and to overcome fertility barriers in crosses involving O.sativa, O.glaberrima parents. Doubled haploid plants produced from several F2 and F3 individuals selected fom japonica X japonica and indica X indica crosses are now in yield or stress trials in 10 countries in WEST-AFRICA. Success was also obtained from progenies selected from japonica X O.Glaben-ima crosses and some Hl regenerants gave up to 100% fertility. In all cases, panicles were cold pre-treated at 10-12°C for 10 to 20 days. Callus were obtained from 21-60 days after induction under 25+/-1°C white plantlets regenerantion under the same incubation temperature was obtained from 30-60 days. A rooting and hardening medium (MS supplemented with MET (MULTI EFFECT TRLKZOL)) increased the survival rate from 20 to about 95% of plantlets transplanted into soil. (Résumé d'auteur)