Résumé
Compartmentalized microfluidics are devices that allow the in vitro reconstitution of neuronal circuits using primary cultures of different neuronal populations. These systems can be used to build thousands of specific neuronal junctions and to study intracellular dynamics using selective markers and live fluorescent reporters. In this work we added a dedicated Micro Electrode Array that fits the microfluidics design to record and control electrical activity of pre-and postsynaptic neurons while simultaneously monitoring intracellular dynamics. Because microfluidics are fluidically isolated, each compartment can be independently manipulated to determine their contribution to network functions.