Résumé
In response to biotic and abiotic stresses, plants have evolved various defence strategies,including the recruitment of health-promoting root-associated microorganisms via a “cry forhelp” mechanism. This selected community of microorganisms is known to help minimize thedamages caused by the stress, for example by modulating plant nutrition or immunity.However, the rules of microbiome assembly following foliar pathogen infection and themechanisms that govern its assembly and function in the diseased host are still poorlyunderstood. In particular, it is not known whether different pathogens can induce differentroot microbiome changes. Using Oryza sativa subsp. japonica cv. Nipponbare, we analysedthe modifications of the root-associated microbiome after host exposure to five different foliarpathogens, including two bacteria (Xanthomonas oryzae and Xanthomonas oryzicola), twofungi (Pyricularia oryzae and Bipolaris oryzae) and one virus (Rice Yellow Mottle Virus). Ricewas grown in a greenhouse on rice field-sampled soil, and inoculated with the respectivepathogens. One week after inoculation, we collected the rhizosphere and analysed thediversity of the microbiome using 16S/18S/gyrB amplicon sequencing approaches. Whetherthe pathogen has an effect on the root microbiome and whether each pathogen affects itdifferently, with common signatures specific to each group of pathogens, will be presented inthis poster