Résumé
The multidrug-resistant bacterium Sm has a gene coding for a cyclophilin (AIL09810.1), whose catalytic site shows strong homology with the humain CypA. This homology allowed us to test the cyclophilin inhibitors, NPSCIs, initially created against humain CypA, as potential inhibitors of the Sm AIL09810.1. In fact, several NPSCIs significantly inhibited the growth of Sm in planktonic form. The NPSCI F909 was tested on Sm growth in biofilm form (data not shown) and on the virulence test model, using the Gm, but not significantly differences with controls were found. This could be explained, in part, by the low expression of AIL09810.1 in the biofilm form. Recombinant Sm AIL09810.1 will be expressed and purified in E. coli to test its PPIase activity and its direct binding to NPSCIs. This results will allow us to elucidate whether the NPSCIs could be used to inhibit the Sm cyclophilin and potentially decrease its virulence.