Résumé
Oil bodies (OB) are naturally structured entities displaying noticeable physico-chemical stability and are of interest for food and non-food applications. To extract OB, effective integrative processes have been developed including optional dehulling/hydration steps/aqueous crushing including rotor-stator and high pressure homogenization followed by centrifugation to collect OB. In addition, alternative processes, including Upstream Dry Fractionation (UDF) have been proposed on some seeds. We propose to compare these integrated processes of OB extraction in terms of yields and physico-chemical composition of OB fraction and of co-products (aqueous phase, centrifugation pellets).Integrated processes of OB extraction including or not UDF step (ball milling) were applied at pilot scale (1L) on three oleoproteagineous seeds (sunflower, oleic sunflower, and rapeseed). Seeds and OB emulsions were characterized in terms of microbial load, lipid content, Fatty Acid (FA) profile, lipid classes, peroxide value and tocopherol content. In addition, the particle size distribution and morphology of the OB emulsions were assessed. The yield/composition in co-products (aqueous phase, centrifugation pellets) were also determined.Integrated processes of extraction of OB led to the collection of 40-60 % of total lipids from the seeds with an impact of the type of seed and of the process (UDF led to more concentrated OB creams). OB had preserved FA profile, preserved content in tocopherol and stable microbial load in range with the initial load in the seed. UDF operation however induced coalescence of part of the OB. Aqueous phases had Dry Matter (DM) ranging between 2-7% and contained very low amount of total lipids. Centrifugation pellets had DM ranging between 30-50 %, this DM was higher when UDF step was added on sunflower and oleic sunflower seeds. This result is part of the OBEINN project which aims at developing sustainable processes of OB extraction for food innovation.