Résumé
Commercial kits based on RNA extraction in a chaotropic medium followed by purification on disposable silica membrane columns fail in yielding substantial amount of RNA when starting from fleshy fruits tissues. The present protocol was designed in order to overcome the strong acidity and dilution inherent to the flesh tissue, as major difficulties in RNA extraction. Nuclear DNA is eliminated upon centrifugation following extraction in a non chaotropic and higly buffered extraction medium, before RNA concentration by LiCl precipitation. This preliminary concentration and purification step allows to load silica column with sub saturating amounts of RNA.