Résumé
Background: Cocoa butter is manufactured as the main by-product from dry fermented cocoa beans. The biochemical composition of this cocoa by-product schows triacylglycerols, di- and mono-glycerides, phospholipids and glycolipids and unsaponifiable matter. During primary post-harvest processing, cocoa beans harbor lipase producing mold species contamination could trigger the accumulation of FFA. High free fatty acids (FFA) content of dry fermented cocoa beans currentely alter physical properties of both fat fraction and chocolate.Aims: This study investigated the improvement of the quality of raw cocoa material sourced from Côte d’Ivoire regarding its FFA concentration.Methods: Fifty dry fermented cocoa beans samples were randomly collected during October 2018 from Aniassué, Akoupé and Yakassé-Attobrou, 3 big cocoa producing regions located in Southeast of Côte d'Ivoire. Dry fermented cocoa beans were sampled on-farm level in the same cocoa producing regions. Contaminating filamentous fungi strains were isolated using Sabouraud chloramphenicol agar incubated at 25°C for 7 days. Morphological identification of fungi isolates was preformed from younger culture. Biochemical study for the determination of lipolytic properties of fungi was carried out using Cazpeck-Dox Agar medium enriched with olive oil as carbon source and rhodamine B as indicator of extracellular lipase production. Extraction, PCR amplification using a specific transcribed spacer primer (ITS4/ITS5) and sequencing of DNA from fungal species were performed for molecular identification.Results: Approximately 154 fungal isolates belonging to 8 genera were found in tested cocoa beans sourced from Côte d’Ivoire. Only Rhizopus oryzae, Absidia corymbifera, Mucor heimalis, Aspergillus niger, Aspergillus tubingensis, Aspergillus aculeatus, Aspergillus flavus, Aspergillus tamari, Aspergillus fumigatus, Nigrospora sphaerica, Curvularia geniculate and Penicillium chrysogenum species exhibited greater extracellular lipase producing ability. Absidia corymbifera, Aspergillus niger, Aspergillus fumigatus and Rhizopus orysae were mostly frequent and predominant species contaminated Ivorian cocoa beans. Dry fermented cocoa beans inoculated with Rhizopus oryzae, Absidia corymbifera or Aspergillus niger recorded higher FFA content above 1.75%.Conclusion: Reducing of the growth of the fungi species growth during cocoa primary post-harvest processing could sustainably produce raw cocoa material with less FFA content. This study clearly indicated that the control of lipolytic fungi contamination in dry fermented cocoa beans could reduce FFA content and sustainably improve the quality of cocoa beans sourced from Côte d’Ivoire, cocoa butter and chocolate produced thereof.