Résumé
Identification of substrates for post-translational modification with ubiquitin and ubiquitin-like molecules is a key in our understanding of the role of this modification in the regulation of biological processes. The ubiquitin-like molecule NEDD8 plays a vital role in cell growth, viability, and development. In this chapter, we describe a method for efficient isolation and detection of NEDDylated proteins in human cells.