Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis

Klaas van Wijk; Jean-Benoît Peltier; Lisa Giacomelli

doi:10.1385/1-59745-227-0:43

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Chapitre d'ouvrage

Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis

Klaas van Wijk, Jean-Benoît Peltier et Lisa Giacomelli

Plant Proteomics, pp.43-48

Humana Press

2007

DOI: https://doi.org/10.1385/1-59745-227-0:43

PMID: 17093301

Résumé

Life Sciences

Vegetal Biology

This chapter describes a simple protocol for large-scale chloroplast purification for proteome analysis. The protocol has not been tested for protein import activity but is optimized for chloroplast proteome yield and purity and minimal protein degradation.

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Détails

Titre: Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis
Créateurs - sans rôle: Klaas van Wijk - Department of Plant Biology
Jean-Benoît Peltier - Department of Plant Biology
Lisa Giacomelli - Department of Plant Biology
Détails de publication: Plant Proteomics, pp.43-48
Éditeur: Humana Press
Nombre de pages: 6
Identifiants: 99141238109311
Unité académique: Institut des Sciences des Plantes de Montpellier - IPSiM
Langue: English
Type de ressource: Book chapter