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Functional Reconstruction of NLRs in HEK293 Cells
Chapitre d'ouvrage

Functional Reconstruction of NLRs in HEK293 Cells

Vincent Compan et Gloria Lopez-Castejon
NLR proteins, pp.217-221
25/05/2016

Résumé

ASC Active caspase-1 HEK293 Inflammasome NLR
Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.

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