Résumé
The study of the organization and dynamics of biological membranes has greatly benefited from the considerable technical advances achieved in the photonics field. Breaking the diffraction limit in optical microscopy to further increase spatial resolution has allowed to define the ultra-structure of the different proteolipid complexes within cellular membranes. Furthermore, the improvements in fluorescence sensitivity have prompted to the analysis of molecular dynamics up to single-molecule level with high temporal resolution. Thanks to all these advances, the concept of tetraspanin-enriched microdomains has been revisited in recent studies that shed new light on tetraspanin dynamics and interactions.