Résumé
As a means of linking recombinant retroviruses to specific cell membrane receptors we used bi-functional antibody complexes. The procedure was employed successfully to infect human cells with ecotropic murine retroviruses via the interaction with MHC class I and class II antigens. The sequential protocol we developed requires less stringent safety constraints than would handling of amphotropic virus stocks. Although its efficiency is still relatively low, the method appears to be general and versatile enough to be adapted to other cell membrane markers.