Résumé
Oxidative stress has been implicated in a wide variety of disease processes. Quantification of oxidative damage products in biological systems is important to understand the role of free radicals in disease states.
Isoprostanes are a family of prostaglandin isomers produced from oxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. The measurement of the F-2-lsoprostanes has been shown to be one of the most accurate approaches to quantifying oxidative damage in vivo. Increased urinary and plasma F-2-Isoprostanes levels have been associated with a number of human diseases.
In recent years, additional related compounds have been discovered like neuroprostanes, derived from docosahexaenoic acid (DHA). DHA is highly enriched in neurons in the brain and is highly susceptible to oxidation. Isofuranes and neurofuranes, free radical-induced peroxidation products of respectively arachidonic acid (AA) and DHA are formed preferentially under conditions of elevated oxygen tension.
We propose a review of mass spectrometry methods developed to quantitate these novel lipid oxidation products as markers of in vivo oxidant stress.